RPB2 Antibody (HL1043) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13698

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1043 expressed in HEK293

Format

Azide and BSA Free
View all RPB2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for RPB2 Antibody (HL1043) - Azide and BSA Free

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698]

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698]

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
RPB2 Antibody (HL1043)

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RPB2 Antibody (HL1043)

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] -

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RPB2 Antibody (HL1043)

Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] -

Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
RPB2 Antibody (HL1043)

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] -

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RPB2 Antibody (HL1043)

Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] -

Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
RPB2 Antibody (HL1043)

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RPB2 Antibody (HL1043)

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RPB2 Antibody (HL1043)

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] -

Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RPB2 Antibody (HL1043)

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] -

Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RPB2 Antibody (HL1043) - Azide and BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPB2

It is suggested that Rpb2, a 140 kDa protein, plays a role is enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.

Alternate Names

DNA-directed RNA polymerase II subunit RPB2, EC 2.7.7, EC 2.7.7.6, hRPB140, hsRPB2, POL2RB, polymerase (RNA) II (DNA directed) polypeptide B (140kD), polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA polymerase II subunit2, RNA polymerase II second largest subunit

Gene Symbol

POLR2B

Additional RPB2 Products

Product Documents for RPB2 Antibody (HL1043) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RPB2 Antibody (HL1043) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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