![Western Blot: RPL22 Antibody [NBP1-06069]](https://resources.rndsystems.com/images/products/RPL22-Antibody-Western-Blot-NBP1-06069-img0007.jpg "Western Blot: RPL22 Antibody [NBP1-06069]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26962690).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RPL22 Antibody
![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]](https://resources.rndsystems.com/images/products/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0006.jpg "Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]")
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]](https://resources.rndsystems.com/images/products/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0003.jpg "Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]")
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]](https://resources.rndsystems.com/images/products/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0004.jpg "Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]")
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]](https://resources.rndsystems.com/images/products/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0005.jpg "Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]")
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069]
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for RPL22 Antibody
Application
Recommended Usage
Immunohistochemistry
3 - 6 ug/mL
Immunohistochemistry-Paraffin
3-6 ug/ml
Peptide ELISA
detection limit 1:16000
Western Blot
1:100-1:2000
Reviewed Applications
Read 1 review rated 4 using NBP1-06069 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RPL22
Alternate Names
EAPHBP15, EBER-associated protein, Epstein-Barr virus small RNA-associated protein, Epstein-Barr-encoded RNA-associated protein, HBP15/L22, heparin-binding protein 15, Heparin-binding protein HBp15, ribosomal protein L22,60S ribosomal protein L22
Gene Symbol
RPL22
UniProt
Additional RPL22 Products
Product Documents for RPL22 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RPL22 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for RPL22 Antibody
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1 Customer Rating
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Application: Western BlotSample Tested: HEK293 cell lysateSpecies: HumanVerified Customer | Posted 11/02/2020Detection of RPL22 protein in HEK293 cell lysate
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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