RPL5 Antibody

Novus Biologicals | Catalog # NBP1-31413

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (98%), Chicken (90%), Porcine (97%), Rabbit (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RPL5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Localization

Cytoplasm, Nucleus, nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPL5 Antibody

Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPL5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: RPL5 Antibody [NBP1-31413]

Immunocytochemistry/ Immunofluorescence: RPL5 Antibody [NBP1-31413]

Immunocytochemistry/Immunofluorescence: RPL5 Antibody [NBP1-31413] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 protein stained by RPL5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.
Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Mouse hind brain. RPL5 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413] - A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE RPL5 antibody dilution: 1:1000
Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413] - A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Gastric CA N87 xenograft, using RPL5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Knockdown Validated: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413]

RPL5-Antibody-Knockdown-Validated-NBP1-31413-img0016.jpg
RPL5 Antibody

Western Blot: RPL5 Antibody [NBP1-31413] -

Western Blot: RPL5 Antibody [NBP1-31413] - A. Methylcellulose colony assay on bone marrow cells from doxycycline treated shRNA Rpl5 & control mice (n=3), (CFU-E, P=0.0003; BFU-E, P=1; GFU-GM, P=0.3); B. FACS analysis of bone marrow cells from shRNA Rpl5 & control mice. Total bone marrow cells from shRNA Rpl5 (B.1) & control (B.2) mice; viable cells were gated for analysis & sorting. We used the cell-surface markers CD71 (Y-axis) & Ter119 (X-axis) to separate mature erythroids & erythroid precursors. The most mature erythroblasts are CD71lowTer119high, while the erythroid precursors are CD71highTer119intermediate cells. In our FACS analysis the gated population comprises CD71highTer119med cells (P4) & CD71highTer119high cells (P5) from shRNA Rpl5 (B.3 & B.5) & control (B4 & B6) mice; the results here are collected from one mouse per group & are representative of 6 independent experiments; in each experiment, cells were collected from 1-3 mice for each group & sorted independently; C. Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice; D. Western Blot analysis of GATA1 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression of GATA1 in the shRNA Rpl5 group compared to the control mice. In these experiments, GAPDH was used as a loading control, & they are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32309614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RPL5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPL5

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Alternate Names

DBA6,60S ribosomal protein L5, MGC117339, ribosomal protein L5

Gene Symbol

RPL5

Additional RPL5 Products

Product Documents for RPL5 Antibody

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Product Specific Notices for RPL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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