RPS10 Antibody - BSA Free

Western Blot: RPS10 Antibody [NBP1-98599]

Western Blot: RPS10 Antibody [NBP1-98599] - Jurkat Cell Lysate 1.0ug/ml, Gel Concentration: 10-20%

Western Blot: RPS10 Antibody - BSA Free [NBP1-98599] -

Core ribosomal protein RPS27A is lost from ribosomes after DSBs. (A) Western blots reveal that RPS27A is depleted in parental HEK cells after electroporation with Cas9‐sgIntron (sgJAK2) RNPs. HEK cells harvested 72 h post dCas9‐sgIntron electroporation served as the negative control. (B) Western blots depict loss of Cas9 protein after Cas9‐sgIntron electroporation. (C) T7 endonuclease 1 (T7E1) assay of JAK2 editing after Cas9‐sgIntron electroporation. Band intensities were calculated using imagej, and percent edited was calculated as 100% × (1 − (1 − fraction cleaved)1/2), where fraction cleaved = (sum of cleavage product intensities)/(sum of uncleaved and cleaved product intensities). (D) Genome editing does not affect JAK2 mRNA abundance. Fold changes were calculated using the2‐ delta delta Ct method with Cas9 without sgIntron (apo Cas9) as the control and GAPDH as the reference gene (n = 3, error bars = standard deviation, P > 0.05, one‐way ANOVA). (E) Western blots show that RPS27A is depleted after DNA DSBs but not after other forms of DNA damage. MMS: methyl methanesulfonate, 0.03%, 1 h. Cas9: Cas9‐sgIntron electroporation, 72 h recovery. H2O2: 500 μm hydrogen peroxide, 1 h. UV: UV irradiation, 20 J·m−2, 6 h recovery. HU: hydroxyurea, 10 mm, 16 h. Etoposide: 5 μm, 16 h. Doxorubicin: 10 μm, 16 h. (F, G) Polysome profiles and western blots of polysome profiling fractions from HEK cells treated with (F) DMSO or (G) 5 μm etoposide for 16 h reveal that RPS27A is lost from ribosomes after DSBs. UV absorbance = UV absorbance at 254 nm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34914197), licensed under a CC-BY license. Not internally tested by Novus Biologicals.