Plant Rubisco ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60142

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Plant tissues (50 - 100 uL)

Sensitivity

3.12 ug/mL

Assay Range

3.12 ug/mL - 800 ug/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Plant

Specificity

This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 20

Scientific Data Images for Plant Rubisco ELISA Kit (Colorimetric)

ELISA: Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142]

ELISA: Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142]

ELISA: Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Plant Rubisco ELISA Kit (Colorimetric)

Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] -

Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - Standard Curve Reference

Kit Contents for Plant Rubisco ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antibody (100 x concentrate)
  • Antibody Diluent
  • HRP-conjugate Diluent
  • HRP-conjugate(100 x concentrate)
  • Instruction manual
  • Sample Diluent
  • Sample Extraction Buffer (25 x concentrate)
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Rubisco

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO. The competitive inhibition reaction is launched between with pre-coated RuBisCO and RuBisCO in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I [Spinacia oleracea], EC 2.1.1.43, rbcL, Ribulose-1

Additional Rubisco Products

Product Documents for Plant Rubisco ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Plant Rubisco ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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