RUNX1 and RUNX1T1 Antibody

Name: RUNX1 and RUNX1T1 Antibody
Brand: Novus Biologicals
SKU: NBP2-59160
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-59160

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all RUNX1 and RUNX1T1 Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RUNX1 and RUNX1T1 Antibody

Western Blot: RUNX1 and RUNX1T1 Antibody [NBP2-59160]

Western Blot: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - Nuclear extracts of SKNO-1 cells (15 ug) were analysed by Western blot using the antibody against AML1-ETO diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (kDa) is shown on the left, the position of the protein of interest is indicated on the right.

Chromatin Immunoprecipitation: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - The IP'd DNA of 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

ELISA: RUNX1 and RUNX1T1 Antibody [NBP2-59160]

ELISA: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against AML1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32,750.

Applications for RUNX1 and RUNX1T1 Antibody

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

4 uL/IP

ELISA

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Serum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RUNX1 and RUNX1T1

Alternate Names

Acute myeloid leukemia 1 protein, AML1, AML1acute myeloid leukemia 1, AML1-EVI-1, AML1T1protein CBFA2T1, CBFA2, CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1, CBFA2T1Cyclin-D-related protein, CDRMGC2796, Core-binding factor subunit alpha-2, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related, DKFZp564B213, Eight twenty one protein, ETOFLJ33145, EVI-1, MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related, PEBP2A2, Protein ETO, Protein MTG8, runt domain, alpha subunit 2, runt-related transcription factor 1, runt-related transcription factor 1; translocated to, 1 (cyclin D-related), Zinc finger MYND domain-containing protein 2, ZMYND2myeloid translocation gene on 8q22

Additional RUNX1 and RUNX1T1 Products

Product Documents for RUNX1 and RUNX1T1 Antibody

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Certificate of Analysis

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Product Specific Notices for RUNX1 and RUNX1T1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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