Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RWDD3 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3. The immunogen is located within the last 50 amino acids of RWDD3. Amino Acid Squence: KEYSALDELQKEFET

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RWDD3 Antibody - BSA Free

Western Blot: RWDD3 AntibodyBSA Free [NBP1-76310]

Western Blot: RWDD3 AntibodyBSA Free [NBP1-76310]

Western Blot: RWDD3 Antibody [NBP1-76310] - Rat kidney tissue lysate with RWDD3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Applications for RWDD3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RWDD3

RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases Ikappabeta sumoylation and stability. In addition, RWDD3 inhibits TNF-alpha-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikappabeta increased stability. RSUME-enhanced sumoylation of Ikappabeta leads to the inhibition of NF-kappabeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

Alternate Names

DKFZP566K023, FLJ51225, RSUME, RWD domain containing 3, RWD domain-containing protein 3, RWD-containing sumoylation enhancer

Gene Symbol

RWDD3

UniProt

NP_056300 (Human)

Additional RWDD3 Products

Product Documents for RWDD3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RWDD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RWDD3 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review RWDD3 Antibody - BSA Free and earn rewards!

Have you used RWDD3 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies