Human S100A9 ELISA Kit (Chemiluminescence)
Novus Biologicals | Catalog # NBP2-62855
Key Product Details
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Specifications
Assay Type
Kit Type
Species
Specificity
Precision
Intra-Assay Precision (Precision within an assay) CV% 9.43%
Inter-Assay Precision (Precision between assays) CV% 9.43%
Recovery for Human S100A9 ELISA Kit (Chemiluminescence)
Recovery
Linearity
Scientific Data Images for Human S100A9 ELISA Kit (Chemiluminescence)
ELISA: Human S100A9 ELISA Kit (Chemiluminescence) [NBP2-62855] -
ELISA: Human S100A9 ELISA Kit (Chemiluminescence) [NBP2-62855] - Standard Curve ReferenceKit Contents for Human S100A9 ELISA Kit (Chemiluminescence)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
Preparation and Storage
Shipping
Stability & Storage
Background: S100A9
Long Name
Alternate Names
Gene Symbol
Additional S100A9 Products
Product Documents for Human S100A9 ELISA Kit (Chemiluminescence)
Certificate of Analysis
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Product Specific Notices for Human S100A9 ELISA Kit (Chemiluminescence)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human S100A9 ELISA Kit (Chemiluminescence)
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Q: Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
A: Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
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Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
A: Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
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Q: Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
A: Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
-
Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
A: Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.