S1P3/EDG-3 Antibody (5E3G9)

Novus Biologicals | Catalog # NBP3-15479

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E3G9 expressed in HEK293
View all S1P3/EDG-3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human S1P3/EDG-3 (Q99500). MATALPPRLQPVRGNETLREHYQYVGKLAGRLKEASEGSTLTTVLFLVICSFIVLENLMVLIAIWKNNKFHNRMYFFIGNLALCDLLAGIAYKVNILMSG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S1P3/EDG-3 Antibody (5E3G9)

Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479]

Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479]

Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479] - Western blot analysis of extracts of various cell lines, using S1P3/EDG-3 Rabbit mAb (NBP3-15479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for S1P3/EDG-3 Antibody (5E3G9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S1P3/EDG-3

EDG3, a Lysophospholipid/Lysosphingolipid Receptor, binds the vasoconstrictor sphingosine-1-phosphate and activates transcription of serum response element-driven reporter genes. EDG3 may regulate angiogenesis and vascular endothelial cell functions. EDG3 has been reported in all tissues, with highest levels in heart, placenta, kidney, and liver. ESTs have been isolated from placenta and germ cell tumor libraries.

Long Name

Endothelial Differentiation Gene 3

Alternate Names

EDG3, LPB3, S1PR3

Gene Symbol

S1PR3

Additional S1P3/EDG-3 Products

Product Documents for S1P3/EDG-3 Antibody (5E3G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for S1P3/EDG-3 Antibody (5E3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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