SA1 Antibody (2E9) - Azide and BSA Free

Novus Biologicals | Catalog # H00010274-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2E9

Format

Azide and BSA Free
View all SA1 Primary Antibodies »

Product Specifications

Immunogen

STAG1 (AAH64699, 1112 a.a. ~ 1221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Reactivity Notes

Human. Other species not tested.

Specificity

STAG1 - stromal antigen 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for SA1 Antibody (2E9) - Azide and BSA Free

Western Blot: SA1 Antibody (2E9) [H00010274-M01]

Western Blot: SA1 Antibody (2E9) [H00010274-M01]

Western Blot: SA1 Antibody (2E9) [H00010274-M01] - STAG1 monoclonal antibody (M01), clone 2E9 Analysis of STAG1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: SA1 Antibody (2E9) [H00010274-M01]

Immunocytochemistry/ Immunofluorescence: SA1 Antibody (2E9) [H00010274-M01]

Immunocytochemistry/Immunofluorescence: SA1 Antibody (2E9) [H00010274-M01] - Analysis of monoclonal antibody to STAG1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SA1 Antibody (2E9) [H00010274-M01]

Immunohistochemistry-Paraffin: SA1 Antibody (2E9) [H00010274-M01]

Immunohistochemistry-Paraffin: SA1 Antibody (2E9) [H00010274-M01] - Analysis of monoclonal antibody to STAG1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml.
Sandwich ELISA: SA1 Antibody (2E9) [H00010274-M01] - Detection limit for recombinant GST tagged STAG1 is approximately 0.03ng/ml as a capture antibody.

Applications for SA1 Antibody (2E9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SA1

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Alternate Names

cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1

Entrez Gene IDs

10274 (Human)

Gene Symbol

STAG1

OMIM

604358 (Human)

UniProt

AAH64699 (Human)

Additional SA1 Products

Product Documents for SA1 Antibody (2E9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SA1 Antibody (2E9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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