SAG dihydrochloride

Tocris Bioscience | Catalog # 6390

Dihydrochloride salt of SAG; Smo receptor agonist
Tocris Bioscience

Key Product Details

Description

Dihydrochloride salt of SAG; Smo receptor agonist
View all Smoothened Receptor Agonists »

Product Description

SAG dihydrochloride is a water-soluble dihydrochloride salt of SAG (Cat. No. 4366). SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons. The compound can be used in protocols for the chemical reprogramming of somatic cells to iPSCs.

Product Specifications for SAG dihydrochloride

Molecular Weight

562.98

Formula

C28H28ClN3OS.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide dihydrochloride

CAS Number

2702366-44-5

PubChem ID

71433770

InChI Key

SWBYSYANHQTINY-CZBZHNTRSA-N

SMILES

ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 56.3 100
DMSO 56.3 100

Preparing Stock Solutions for SAG dihydrochloride

The following data is based on the product molecular weight 562.98.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.78 mL 8.88 mL 17.76 mL
5 mM 0.36 mL 1.78 mL 3.55 mL
10 mM 0.18 mL 0.89 mL 1.78 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 9 publications citing the usage of this product.

Product Documents for SAG dihydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SAG dihydrochloride

For research use only

Citations for SAG dihydrochloride

Customer Reviews for SAG dihydrochloride (1)

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  • MUCH better than the others!!
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: stem cells
    Verified Customer | Posted 08/28/2020
    This SAG performs the BEST for stem cell differentiation to neuronal lineages. Other brands do not work. We use it at 1uM after dual SMAD inhibition.

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