Catalog Number: 4366
Chemical Name: 3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
Biological Activity
Potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine action at the Smo receptor. Potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM). Induces pathway activation independently of Ptch proteins. Putative inhibitor of a cellular component required for Hedgehog signaling. Enhances neuronal differentiation of iPSCs into dopaminergic neurons.
Technical Data
  • M.Wt:
    490.06
  • Formula:
    C28H28ClN3OS
  • Solubility:
    Soluble to 100 mM in DMSO and to 50 mM in 1eq. HCl
  • Purity:
    >99%
  • Storage:
    Store at -20°C
  • CAS No:
    912545-86-9
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Small molecule modulation of Smoothened activity.
    Chen et al.
    Proc.Natl.Acad.Sci.USA., 2002;99:14071
  2. Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage.
    Mak et al.
    Stem Cells Int., 2012;2012:140427
  3. A small molecule that binds Hedgehog and blocks its signaling in human cells.
    Stanton et al.
    Nat.Chem.Biol., 2009;5:154
Citations:

The citations listed below are publications that use Tocris products. Selected citations for SAG include:

2 Citations: Showing 1 - 2
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  1. Transcriptional comparison of human induced and primary midbrain dopaminergic neurons.
    Authors: Xia Et al.
    Sci.Rep. 2016;6:20270
  2. Accumulation of the Vitamin D Precursor Cholecalciferol Antagonizes Hedgehog Signaling to Impair Hemogenic Endothelium Formation.
    Authors: Cortes Et al.
    Nat Chem Biol 2015;5:471

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