SALL4 Antibody

Novus Biologicals | Catalog # NB100-60559

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SALL4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DQSKKGKGKPQN corresponding to internal region according to Uniprot Mouse NP_780512.2, NP_958797.2, NP_958798.2.

Epitope

DQSKKGKGKPQN

Specificity

This antibody is expected to recognize isoform a (NP_780512.2) and isoform b (NP_958797.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SALL4 Antibody

Western Blot: SALL4 Antibody [NB100-60559]

Western Blot: SALL4 Antibody [NB100-60559]

Western Blot: SALL4 Antibody [NB100-60559] - Western blot analysis of SALL4 using NB100-60559 at 0.1ug/ml in rat testis lysate (35ug protein in RIPA buffer).
Western Blot: SALL4 Antibody [NB100-60559]

Western Blot: SALL4 Antibody [NB100-60559]

Western Blot: SALL4 Antibody [NB100-60559] - (0.1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SALL4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in rat spleen and testis lysates (calculated MW of 66.2 kDa band according to NP_958797.2). Additional bands of unknown identity was also consistently observed at 28+24 kDa. These bands were successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SALL4

Sall4 is a probable transcription factor. Defects in Sall4 are the cause of Okihiro syndrome; also known as Duane radial ray syndrome (DRRS). It is a disorder characterized by the association of forearm malformations with Duane retraction syndrome. Sall4 is also involved in forelimb and heart development in mice.

Long Name

Sal-like 4

Alternate Names

DRRS, HSAL4, ZNF797

Entrez Gene IDs

99377 (Mouse)

Gene Symbol

SALL4

UniProt

NP_780512.2 (Mouse)

Additional SALL4 Products

Product Documents for SALL4 Antibody

Certificate of Analysis

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Product Specific Notices for SALL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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