SAP30 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56769

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SAP30 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: NEKDTLTYFIYSVKNDKNKSDLKVDSGVH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAP30 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SAP30 Antibody [NBP2-56769]

Immunocytochemistry/ Immunofluorescence: SAP30 Antibody [NBP2-56769]

Immunocytochemistry/Immunofluorescence: SAP30 Antibody [NBP2-56769] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
SAP30 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SAP30 Antibody - BSA Free [NBP2-56769]

Chromatin Immunoprecipitation-exo-Seq: SAP30 Antibody - BSA Free [NBP2-56769]

ChIP-Exo-Seq composite graph for Anti-SAP30 (NBP2-56769) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for SAP30 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAP30

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq]

Alternate Names

30 kDa Sin3-associated polypeptide, histone deacetylase complex subunit SAP30, Sin3 corepressor complex subunit SAP30, Sin3A-associated protein, 30kDa, Sin3-associated polypeptide p30, sin3-associated polypeptide, 30 kDa, Sin3-associated polypeptide, 30kDa

Gene Symbol

SAP30

Additional SAP30 Products

Product Documents for SAP30 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SAP30 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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