Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Sarcosine oxidase Primary Antibodies »

Product Specifications

Immunogen

Sarcosine oxidase [Microbial] (Uniprot: Q46337)

Reactivity Notes

Cross reactivity against Sarcosine oxidase from other sources is unknown.

Specificity

Cross reactivity against Sarcosine oxidase from other sources is unknown.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Sarcosine oxidase Antibody - BSA Free

Western Blot: Sarcosine oxidase Antibody [NB600-649]

Western Blot: Sarcosine oxidase Antibody [NB600-649]

Western Blot: Sarcosine oxidase Antibody [NB600-649] - Detection of Sarcosine Oxidase. Samples of 1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Sarcosine Oxidase. Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat 1:10K 1.5 hr RT in and imaged on the VersaDoc imaging system.
Western Blot: Sarcosine oxidase Antibody [NB600-649]

Western Blot: Sarcosine oxidase Antibody [NB600-649]

Western Blot: Sarcosine oxidase Antibody [NB600-649] - Analysis using HRP conjugate of NB600-649. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.

Applications for Sarcosine oxidase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by dot blot and western blot and is suitable for ELISA assay against 1.0 ug of Sarcosine Oxidase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Sarcosine oxidase

Sarcosine Oxidase catalyzes the oxidative demethylation of sarcosine. It can also oxidize other secondary amino acids such as N methyl L alanine, L pipecolic acid and L proline.

UniProt

Q46337 (Human)

Additional Sarcosine oxidase Products

Product Documents for Sarcosine oxidase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Sarcosine oxidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Sarcosine oxidase Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Sarcosine oxidase Antibody - BSA Free and earn rewards!

Have you used Sarcosine oxidase Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies