SARS-CoV-2 nsp1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30993

Novus Biologicals
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SARS-CoV-2 nsp1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant SARS-CoV-2 NSP1(Met1-Gly180)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 nsp1 Antibody - BSA Free

SARS-CoV-2 nsp1 Antibody [NBP3-30993] -

Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 nsp1 Antibody at dilution of 1:1000

Applications for SARS-CoV-2 nsp1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:4000~1:8000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp1

The SARS-CoV-2 Membrane protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). The membrane protein is the most abundant of the four structural proteins, which also includes the envelope protein, spike protein, and nucleocapsid protein (1,2). The membrane protein is synthesized as a 222 amino acid protein with a theoretical molecular weight of 25.1 kDa (2,3). Furthermore, the membrane protein of SARS-CoV-2 has 90.5% sequence identity and 98.2% sequence similarity to that of the membrane protein of SARS-CoV (2). Structurally, the membrane protein is a type III transmembrane glycoprotein containing a glycosylated N-terminal ectodomain, three transmembrane domains, and a large C-terminal that extends into the viral particle (2,4). The membrane protein exists as a dimer and is either in long or compact conformation, which contributes to the curvature of the viral membrane (2,4). The main functions of the membrane protein are for viral assembly and contributing to the shape and structural integrity of the viral envelope (1,2). Additional functions show a role in inducing apoptosis as well as interactions with the spike and nucleocapsid proteins (1,2). The membrane-spike protein interaction is required for maintaining the spike protein within the ER-Golgi intermediate compartment (1). The membrane protein binding with the nucleocapsid protein is responsible for nucleocapsid stabilization and viral assembly (1).

References

1. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

2. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

3. Uniprot (P0DTC5)

4. J Alsaadi, E. A., & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144

Alternate Names

Non-structural protein 1, nsp1, ORF1a polyprotein, ORF1ab polyprotein, pp1a, Replicase polyprotein 1a

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp1 Products

Product Documents for SARS-CoV-2 nsp1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 nsp1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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