SARS-CoV-2 nsp4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-07050

Novus Biologicals
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SARS-CoV-2 nsp4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV-2 (COVID-19) NSP4 protein. The immunogen is located within 50-100 amino acids of the SARS-CoV-2 (COVID-19) NSP4 protein.

Reactivity Notes

Predicted reactivity based on immunogen sequence: SARS-CoV NSP4 protein: (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 nsp4 Antibody - BSA Free

ELISA: SARS-CoV-2 nsp4 Antibody - BSA Free [NBP3-07050]

ELISA: SARS-CoV-2 nsp4 Antibody - BSA Free [NBP3-07050]

ELISA: SARS-CoV-2 nsp4 Antibody [NBP3-07050] - Antibodies: SARS-CoV-2 (COVID-19) NSP4 Anbody. A direct ELISA was performed using immunogen peptide (9175P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP4 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.

Applications for SARS-CoV-2 nsp4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: SARS-CoV-2 nsp4

SARS-CoV-2 Nonstructural Protein 4 (NSP4) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP4 is 500 amino acids (aa) with a theoretical molecular weight of 56.2 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP4 has 80% sequence identity and 95% sequence similarity (1). Structurally, SARS-CoV-2 NSP4 protein has four transmembrane domains (3). SARS-CoV-2 interactome studies revealed that NSP4 interacts with the TIM (translocase of the inner membrane) complex, indicating an association with the mitochondria (2). Additionally, SARS-CoV-2 NSP4 is shown to interact with NSP3 and this interaction is required for viral replication (1). Specifically, the NSP3-NSP4 interaction is responsible for double membrane vesicle formation (3).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M.,... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

Alternate Names

Corona_NSP4_C, Coronavirus nonstructural protein 4 C-terminus, Non-structural protein 4, nsp4

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp4 Products

Product Documents for SARS-CoV-2 nsp4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 nsp4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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