SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05727

Novus Biologicals
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, ELISA, Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Human IgG Clone # B003N

Format

Azide and BSA Free
View all SARS-CoV-2 Spike RBD Primary Antibodies »

Product Specifications

Immunogen

SARS-CoV-2 Spike RBD recombinant protein

Clonality

Monoclonal

Host

Human

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free

Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N)(RBD)Azide and BSA Free [NBP3-05727]

Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N)(RBD)Azide and BSA Free [NBP3-05727]

Western Blot: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 Spike-RBD Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.
ELISA: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free [NBP3-05727]

ELISA: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free [NBP3-05727]

ELISA: SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) [NBP3-05727] - ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein. Antibodies: SARS-CoV-2 Spike RBD Antibody, NBP3-05727. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15min, 100 uL/well. Stop: Stop buffer 50 uL/well.

Applications for SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

1:40000

ELISA

1:80000

Western Blot

1:4000-5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 Spike RBD

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Alternate Names

2019-nCoV Peplomer protein, 2019-nCoV S Protein, 2019-nCoV Spike, COVID-19 Spike, E2, Human coronavirus spike glycoprotein, Peplomer protein, S glycoprotein, S protein, SARS-CoV-2, SARS-COV-2 E2, SARS-COV-2 Peplomer protein, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein, Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein, Spike, Spike glycoprotein, Spike S1, Spike S1 RBD, surface glycoprotein

Gene Symbol

S

Additional SARS-CoV-2 Spike RBD Products

Product Documents for SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Spike RBD Antibody (B003N) - (RBD) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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