SATB2 Antibody (MSVA-702R)

Novus Biologicals | Catalog # NBP3-57233

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-702R
View all SATB2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2 Swissprot Q9UPW6

Localization

Nuclear

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A strong nuclear staining should be seen in virtually all columnar epithelial cells..

Negative Control
Negitve control:Colon: SATB2 staining should be absent in stromal and smooth muscle cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SATB2 Antibody (MSVA-702R)

SATB2 Antibody (MSVA-702R)

Immunohistochemistry-Paraffin:SATB2 Antibody (MSVA-702R)NBP3-57233

Colon Colorectal adenocarcinoma showing strong SATB2 immunostaining of all tumor cells.
SATB2 Antibody (MSVA-702R)

Immunohistochemistry-Paraffin:SATB2 Antibody (MSVA-702R)NBP3-57233

A strong nuclear SATB2 immunostaining is seen in all epithelial cells of the normal colorectal mucosa.
SATB2 Antibody (MSVA-702R)

Immunohistochemistry-Paraffin:SATB2 Antibody (MSVA-702R)NBP3-57233

A strong nuclear SATB2 immunostaining is seen in all epithelial cells of the normal colorectal mucosa.

Applications for SATB2 Antibody (MSVA-702R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SATB2

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Alternate Names

DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2

Gene Symbol

SATB2

Additional SATB2 Products

Product Documents for SATB2 Antibody (MSVA-702R)

Certificate of Analysis

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Product Specific Notices for SATB2 Antibody (MSVA-702R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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