SCARA3 Antibody - BSA Free

Immunohistochemistry: SCARA3 Antibody - BSA Free [NBP2-13286]

Staining of human prostate shows strong cytoplasm granular positivity in smooth muscle cells.

Immunohistochemistry: SCARA3 Antibody - BSA Free [NBP2-13286]

Staining of human kidney shows strong cytoplasm granular positivity in cells in tubules.

Immunohistochemistry: SCARA3 Antibody - BSA Free [NBP2-13286]

Staining of human fallopian tube shows strong cytoplasm granular positivity.

Immunohistochemistry: SCARA3 Antibody - BSA Free [NBP2-13286]

Staining of human small intestine shows strong cytoplasm granular positivity in glandular cells.

Western Blot: SCARA3 Antibody - BSA Free [NBP2-13286]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Western Blot: SCARA3 Antibody - BSA Free [NBP2-13286] -

SCARA3 inhibits proliferation of lung cancer in vivo. A Western blot analysis for Flag-SCARA3 expression in indicated H1299 cells. B Tumors derived from indicated H1299 cells at end point (n = 5 mice per group). C Average tumor weight of indicated group at the endpoint of the experimenth. D Growth curves of tumors derived from indicated H1299 cells in mice. Data are shown as mean +/- SD. ***, P < 0.001, two-way ANOVA. E Western blot analysis for shSCARA3 expression in indicated A549 cells. F Tumors derived from the indicated A549 cells at end point (n = 5 mice per group). G Average tumor weight of the indicated group at the endpoint of the experiment. H Growth curves of tumors derived from indicated A549 cells in mice. Data are shown as mean +/- SD. ***, P < 0.001, two-way ANOVA. I and J Tumor sections from mice injected with indicated H1299 and A549 cells were stained with hematoxylin-eosin. Immunostained sections were stained with SCARA3 and Ki67. Scale bar = 100 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35578316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SCARA3 Antibody - BSA Free [NBP2-13286] -

SCARA3 inhibits proliferation of lung cancer in vivo. A Western blot analysis for Flag-SCARA3 expression in indicated H1299 cells. B Tumors derived from indicated H1299 cells at end point (n = 5 mice per group). C Average tumor weight of indicated group at the endpoint of the experimenth. D Growth curves of tumors derived from indicated H1299 cells in mice. Data are shown as mean +/- SD. ***, P < 0.001, two-way ANOVA. E Western blot analysis for shSCARA3 expression in indicated A549 cells. F Tumors derived from the indicated A549 cells at end point (n = 5 mice per group). G Average tumor weight of the indicated group at the endpoint of the experiment. H Growth curves of tumors derived from indicated A549 cells in mice. Data are shown as mean +/- SD. ***, P < 0.001, two-way ANOVA. I and J Tumor sections from mice injected with indicated H1299 and A549 cells were stained with hematoxylin-eosin. Immunostained sections were stained with SCARA3 and Ki67. Scale bar = 100 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35578316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SCARA3 Antibody - BSA Free [NBP2-13286] -

Overexpression of SCARA3 downregulates Epithelial-Mesenchymal Transition (EMT). A Migration of H1299 and A549 cells determined with Transwell assays. B H1299 and A549 invasion ability examined by Matrigel Transwell invasion assay. Quantitative results of migration and invasion assays are shown below. Data are presented as mean +/- SD of three independent experiments. ***, P < 0.001. C Flag-tagged SCARA3 was expressed in H1299 cells. Protein levels of beta -catenin, vimentin, and MMP9 were examined by Western blot using their specific antibodies. D Immunofluorescent staining of fixed H1299 cells with anti-Flag and beta -catenin antibodies. Nuclei were stained with DAPI. Scale bar = 20 μm. E Flag-tagged SCARA3 was expressed in A549 cells. Protein levels of beta -catenin, vimentin, and MMP9 were examined by Western blot using their specific antibodies. F Immunofluorescent staining of fixed A549 cells with anti-Flag and beta -catenin antibodies. Nuclei were stained with DAPI. Scale bar = 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35578316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SCARA3 Antibody - BSA Free [NBP2-13286] -

SCARA3 is aberrantly downregulated in lung cancer. A Expression of SCARA3 mRNA in 10 primary cancer types from TCGA. Data are shown as mean +/- SD. ns, not significant; ***, P < 0.001, two-tailed Student’s t-test. B SCARA3 gene is located on the short arm of chromosome 8 at position 8q21.1. C Alteration frequency of SCARA3 gene in 10 cancer types from TCGA. D Kaplan-Meier analysis of survival according to SCARA3 in lung cancer, breast cancer, and head and neck cancer patients. P values are from a Log-rank test. E, F SCARA3 protein was immunohistochemically stained with brown color in the cytoplasm and/or nuclei of lung cancer tissues. Scale bar = 100 μm. G, H Expression levels of SCAR3 proteins (G) mRNAs (H) were determined by Western blot and RT- qPCR, respectively, using specific anti-SCARA3 antibodies and SCARA3-specific primers in various lung cancer cell lines and adjacent normal cell lines. Data are presented as mean +/- SD of three independent experiments. **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35578316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SCARA3 Antibody - BSA Free [NBP2-13286] -

Overexpression of SCARA3 downregulates Epithelial-Mesenchymal Transition (EMT). A Migration of H1299 and A549 cells determined with Transwell assays. B H1299 and A549 invasion ability examined by Matrigel Transwell invasion assay. Quantitative results of migration and invasion assays are shown below. Data are presented as mean +/- SD of three independent experiments. ***, P < 0.001. C Flag-tagged SCARA3 was expressed in H1299 cells. Protein levels of beta -catenin, vimentin, and MMP9 were examined by Western blot using their specific antibodies. D Immunofluorescent staining of fixed H1299 cells with anti-Flag and beta -catenin antibodies. Nuclei were stained with DAPI. Scale bar = 20 μm. E Flag-tagged SCARA3 was expressed in A549 cells. Protein levels of beta -catenin, vimentin, and MMP9 were examined by Western blot using their specific antibodies. F Immunofluorescent staining of fixed A549 cells with anti-Flag and beta -catenin antibodies. Nuclei were stained with DAPI. Scale bar = 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35578316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.