SEMA3D Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-85517
![Immunohistochemistry-Paraffin: SEMA3D Antibody [NBP1-85517]](https://resources.rndsystems.com/images/products/SEMA3D-Antibody-Immunohistochemistry-Paraffin-NBP1-85517-img0006.jpg "Immunohistochemistry-Paraffin: SEMA3D Antibody [NBP1-85517]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (91%), Rat (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry-Paraffin, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SEMA3D Antibody - BSA Free
Immunohistochemistry-Paraffin: SEMA3D Antibody [NBP1-85517]
Immunohistochemistry-Paraffin: SEMA3D Antibody [NBP1-85517] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry: SEMA3D Antibody - BSA Free [NBP1-85517] -
Abundant expression of SEMA3D and PDE1A proteins in layers of muscularis propria and mucosa in inflamed CD specimens. (A) Immunohistochemistry (IHC) images showing representative colon specimens from patients with CD and UC, including non-inflammatory and inflammatory samples. Staining demonstrates expression of SEMA3D and PDE1A at a magnification of ×20. The scale bar corresponds to 40 μm. (B,C) Graphs presenting the quantification of staining (percentage of DAB-positive tissue) in niBD, iUC, and iCD cohorts (niBD, n = 4; iUC, n = 7; iCD, n = 15). Statistical analysis was performed using Fisher’s LSD test. Nonsignificant p-values (>0.05) are denoted. Asterisks (*) indicate statistical significance levels: *p ≤ 0.05, **p ≤ 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37727374), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SEMA3D Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 5 using NBP1-85517 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Semaphorin 3D
Alternate Names
coll-2, Collapsin 2, Sema-Z2, SEMA3D, Semaphorin-3D
Gene Symbol
SEMA3D
Additional Semaphorin 3D Products
Product Documents for SEMA3D Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SEMA3D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for SEMA3D Antibody - BSA Free
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Customer Reviews for SEMA3D Antibody - BSA Free (1)
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Application: Western BlotSample Tested: Adult mouse brain tissueSpecies: MouseVerified Customer | Posted 11/01/2016Western blot for SEMA3D in mouse brain tissue
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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