Septin-7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85731

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Septin-7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Septin-7 Antibody - BSA Free

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-SEPT7 antibody NBP1-85731 (A) shows similar protein distribution across tissues to independent antibody NBP1-85730 (B).
Immunocytochemistry/ Immunofluorescence: Septin-7 Antibody [NBP1-85731]

Immunocytochemistry/ Immunofluorescence: Septin-7 Antibody [NBP1-85731]

Immunocytochemistry/Immunofluorescence: Septin-7 Antibody [NBP1-85731] - Staining of human cell line A-431 shows localization to nucleoli, plasma membrane & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Western Blot: Septin-7 Antibody [NBP1-85731]

Western Blot: Septin-7 Antibody [NBP1-85731]

Western Blot: Septin-7 Antibody [NBP1-85731] - Analysis in human cell line U-2 OS.
Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human testis shows moderate positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731]

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Septin-7 Antibody - BSA Free Western Blot: Septin-7 Antibody - BSA Free [NBP1-85731]

Western Blot: Septin-7 Antibody - BSA Free [NBP1-85731]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Septin-7 Antibody - BSA Free Western Blot: Septin-7 Antibody - BSA Free [NBP1-85731]

Western Blot: Septin-7 Antibody - BSA Free [NBP1-85731]

Analysis in human cell line U-2 OS.

Applications for Septin-7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Septin-7

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

CDC10 cell division cycle 10 homolog, CDC10 cell division cycle 10 homolog (S. cerevisiae), CDC10 protein homolog, CDC10S. cerevisiae, homolog), Nbla02942, septin 7, septin-7

Gene Symbol

SEPTIN7

Additional Septin-7 Products

Product Documents for Septin-7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Septin-7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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