Serine racemase Antibody (JE65-58)

Novus Biologicals | Catalog # NBP3-32943

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE65-58 expressed in HEK293
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Product Specifications

Immunogen

Recombinant protein within Human Serine racemase aa 1-100 / 340. (Uniprot: Q9GZT4)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serine racemase Antibody (JE65-58)

Serine racemase Antibody (JE65-58)

Western Blot: Serine racemase Antibody (JE65-58) [NBP3-32943]

Western Blot: Serine racemase Antibody (JE65-58) [NBP3-32943] - Western blot analysis of Serine racemase on different lysates with Rabbit anti-Serine racemase antibody (NBP3-32943) at 1/1,000 dilution.

Lane 1: LNCaP cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: HepG2 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32943) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Serine racemase Antibody (JE65-58)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serine racemase

Serine racemase catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis

Alternate Names

D-serine ammonia-lyase, D-serine dehydratase, EC 4.3.1.17, EC 4.3.1.18, EC 5.1.1.18, ILV1, ISO1, L-serine ammonia-lyase, L-serine dehydratase, serine racemase

Gene Symbol

SRR

Additional Serine racemase Products

Product Documents for Serine racemase Antibody (JE65-58)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Serine racemase Antibody (JE65-58)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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