Serotonin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA1894

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Platelets, Serum, Urine (25 uL)

Sensitivity

6.2 ng/mL

Assay Range

10.2 to 2,500 ng/mL
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Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Description

Serotonin ELISA Kit is an enzyme immunoassay for the quantitative determination of Serotonin in serum, urine and platelets.

Precision

Intra-Assay Precision (Precision within an assay) Urine: 6.5%, Serum: 4.65%

Inter-Assay Precision (Precision between assays) Urine: 7.65%, Serum: 6.0%

Recovery for Serotonin ELISA Kit (Colorimetric)

Recovery

Urine: 94%, Serum: 97%

Scientific Data Images for Serotonin ELISA Kit (Colorimetric)

ELISA: Serotonin ELISA Kit (Colorimetric) [KA1894]

ELISA: Serotonin ELISA Kit (Colorimetric) [KA1894]

ELISA: Serotonin ELISA Kit [KA1894] - Standard curve
ELISA: Serotonin ELISA Kit (Colorimetric) [KA1894]

ELISA: Serotonin ELISA Kit (Colorimetric) [KA1894]

ELISA: Serotonin ELISA Kit (Colorimetric) [KA1894] - Image from verifed customer review.

Kit Contents for Serotonin ELISA Kit (Colorimetric)

  • Acylation Buffer: Tris buffer with non-mercury preservative, ready to use.
  • Acylation Reagent: Acylation reagent in dimethylsulfoxide, ready to use.
  • Enzyme Conjugate: Ready to use, goat anti-rabbit immunoglobulins conjugated with peroxidase.
  • Reaction Tubes*): Ready to use, reaction tubes in a resealable pouch.
  • Serotonin Antiserum: Ready to use, rabbit anti-serotonin antibody, blue coloured.
  • Serotonin Microtiter Strips: Ready to use, antigen pre-coated microwell plate in a resealable pouch with desiccant.
  • Stop Solution: Ready for use, containing 0.25 M sulfuric acid. Hazards identification: H290 May be corrosive to metals. H314 Causes severe skin burns and eye damage
  • Substrate: Ready to use, chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide
  • Wash Buffer Concentrate (50x): Buffer with a non-ionic detergent and physiology pH.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Serotonin

In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Alternate Names

5-HT, 5-hydroxytryptamne

Additional Serotonin Products

Product Documents for Serotonin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Serotonin ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Serotonin ELISA Kit (Colorimetric)

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  • Serotonin ELISA Kit (Colorimetric)
    Name: Anonymous
    Sample Tested: Positive Controls (Included), Animal Component Free Media and RPMI + 10% FBS
    Verified Customer | Posted 01/23/2019
    Serotonin ELISA Kit (Colorimetric) KA1894

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FAQs for Serotonin ELISA Kit (Colorimetric)

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  • Q: Can this kit be used with rat serum/plasma?

    A: Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.

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