The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
Serpin G1/C1 Inhibitor Antibody (18) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-89738
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Sandwich ELISA Detection, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 18 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Serpin G1/C1 Inhibitor (Accession#: NP_000053.2; Met 1-Ala 500).
Specificity
No cross-reactivity in ELISA with: Human SerpinA1 / A1AT Human SerpinA3 / SERPINA3 / AACT Human SerpinA5 / PROCI Human SerpinC1 /SERPINC1/ Antithrombin-III Human SerpinD1 / SERPIND1 / HC2 Human SerpinF1 / SERPINF1 / PEDF Human SerpinF2 / SERPINF2 Human SerpinI1 / SERPINI1 / Protease inhibitor 12
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Serpin G1/C1 Inhibitor Antibody (18) - Azide and BSA Free
Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738]
Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDaImmunoprecipitation: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738]
Immunoprecipitation: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SERPING1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-SERPING1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 55 kDa Observed band size: 100 kDaApplications for Serpin G1/C1 Inhibitor Antibody (18) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Serpin G1/C1 Inhibitor
Alternate Names
C1 Inhibitor
Gene Symbol
SERPING1
Additional Serpin G1/C1 Inhibitor Products
Product Documents for Serpin G1/C1 Inhibitor Antibody (18) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Serpin G1/C1 Inhibitor Antibody (18) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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