SESN1 Antibody [FITC]

Novus Biologicals | Catalog # NBP1-44994

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all SESN1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to unique epitope on SESN1. The SESN1 peptide was selected from N-terminal, amino acids 1-50. This peptide was covalently modified post-synthetically to achieve desired antigenicity.

Reactivity Notes

This antibody is reactive in Human, Mouse and Rat. Other species not yet tested

Specificity

This antibody is specific to SESN1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for SESN1 Antibody [FITC]

Application
Recommended Usage

ELISA

< 1:10000

Western Blot

>1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SESN1

Sestrin 1(SESN1), located on rat chromosome 20q13, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Sestrin 1 is implicated in the inhibition of cell growth. It has been reported that Sestrin 1 activates AMP-responsive protein kinase (AMPK) and targets it to phosphorylate TSC2, a tumor suppressor. Phosphorylation of TSC2 stimulates its GAP activity which inhibits the activity of mTOR, a positive regulator of cell growth, thus leading to inhibition of cell proliferation and growth (4). Inhibition of mRNAs from sestrin 1 and 3 genes has been shown to substantially increase intracellular ROS levels. This increase in ROS is linked to Ras-induced mutagenesis which is one of the most common events in cancer (5). Sestrin 1 was also identified as a candidate gene for heterotaxia in humans (6). Recent studies have identified that AMPK is an essential mediator of tumor suppressor LKB1 and is suppressed in cancer cells (7). Since activity of AMPK is regulated by sestrin 1, this protein could be a promising target for cancer treatment and therapy. Sestrin 1 is approximately a 66.1kDa protein in humans (551 amino acids) and 55.4kDa protein in mouse and rat (462 amino acids).

Alternate Names

MGC138241, MGC142129, p53-regulated protein PA26, PA26p53 activated gene 26, SEST1p53 regulated PA26 nuclear protein, sestrin 1, sestrin-1

Gene Symbol

SESN1

Additional SESN1 Products

Product Documents for SESN1 Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SESN1 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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