Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SET1B Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human SET domain containing 1B using the numbering given in entry NP_055863.1 (GeneID 23067).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SET1B Antibody - BSA Free (NBP1-28684) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SET1B Antibody - BSA Free

Immunohistochemistry: SET1B Antibody [NBP1-28684]

Immunohistochemistry: SET1B Antibody [NBP1-28684]

Immunohistochemistry: SET1B Antibody [NBP1-28684] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSET1B used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28684]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28684]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28684] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28684]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28684]

Immunohistochemistry-Paraffin: SET1B Antibody [NBP1-28684] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: SET1B Antibody [NBP1-28684]

Immunoprecipitation: SET1B Antibody [NBP1-28684]

Immunoprecipitation: SET1B Antibody [NBP1-28684] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SET1B antibody NBP1-28684 used for IP at 10 ug/mg lysate. SET1B was also immunoprecipitated by rabbit anti-SET1B antibody NBP1-28685, which recognizes a downstream epitope. For blotting immunoprecipitated SET1B, NBP1-28684 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SET1B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SET1B

hSET1B is a histone methyltransferase that associates in a complex with the non-catalytic subunits CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. These five non-catalytic subunits are also part of the Set1A complex. The SET1B complex has been shown to catalyze the tri-methylation of 'Lys-4' on histone H3. SET1B has been shown to localize to sites independent of SET1A complexes indicating non-redundant functions between SET1B and SET1A complexes. hSET1B include SET domain-containing protein 1B, lysine N-methyltransferase 2G, SETD1B, KMT2G, SET1B, and KIAA1076.

Alternate Names

EC 2.1.1, FLJ20803, histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076EC 2.1.1.43, KMT2GSET1B, Lysine N-methyltransferase 2G, SET domain containing 1B, SET domain-containing protein 1B, Set1B

Entrez Gene IDs

23067 (Human)

Gene Symbol

SETD1B

UniProt

NP_055863.1 (Human)

Additional SET1B Products

Product Documents for SET1B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SET1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SET1B Antibody - BSA Free

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Protocols

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