SETD8 Antibody (5M9P5)

Novus Biologicals | Catalog # NBP3-16339

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5M9P5 expressed in HEK293
View all SETD8 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SETD8 (Q9NQR1). MGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETD8 Antibody (5M9P5)

Western Blot: SETD8 Antibody (5M9P5) [NBP3-16339]

Western Blot: SETD8 Antibody (5M9P5) [NBP3-16339]

Western Blot: SETD8 Antibody (5M9P5) [NBP3-16339] - Western blot analysis of extracts of various cell lines, using SETD8 Rabbit mAb (NBP3-16339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
SETD8 Antibody (5M9P5)

Western Blot: SETD8 Antibody (5M9P5) [NBP3-16339] -

Western Blot: SETD8 Antibody (5M9P5) [NBP3-16339] - Western blot analysis of lysates from wild type(WT) and SETD8 knockdown (KD) 293T cells, using [KD Validated] SETD8 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Enhanced Kit.
Exposure time: 30s.

Applications for SETD8 Antibody (5M9P5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SETD8

Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20'monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression.Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNAduring mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substratecompared to free histones

Long Name

N-lysine methyltransferase KMT5A

Alternate Names

H4-K20-HMTase KMT5A, KMT5A, PR-Set7, PR/SET07, PRSET7, SET07, SET8

Gene Symbol

KMT5A

Additional SETD8 Products

Product Documents for SETD8 Antibody (5M9P5)

Certificate of Analysis

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Product Specific Notices for SETD8 Antibody (5M9P5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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