Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SETD8 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human SETD8 (Accession#: NP_065115.3; Lys195-His352)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SETD8 Antibody - Azide and BSA Free

Western Blot: SETD8 Antibody [NBP2-99864]

Western Blot: SETD8 Antibody [NBP2-99864]

Western Blot: SETD8 Antibody [NBP2-99864] - Anti-SETD8 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HCT-116 Whole Cell Lysate. Lane C: U87-MG Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa
Immunoprecipitation: SETD8 Antibody [NBP2-99864]

Immunoprecipitation: SETD8 Antibody [NBP2-99864]

Immunoprecipitation: SETD8 Antibody [NBP2-99864] - SETD8 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 ul anti-SETD8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-SETD8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 54 kDa

Applications for SETD8 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETD8

Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20'monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression.Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNAduring mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substratecompared to free histones

Long Name

N-lysine methyltransferase KMT5A

Alternate Names

H4-K20-HMTase KMT5A, KMT5A, PR-Set7, PR/SET07, PRSET7, SET07, SET8

Gene Symbol

KMT5A

Additional SETD8 Products

Product Documents for SETD8 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SETD8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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