SGK1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98778

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SGK1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SGK1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SGK1 Antibody - Azide and BSA Free

Immunoprecipitation: SGK1 Antibody [NBP2-98778]

Immunoprecipitation: SGK1 Antibody [NBP2-98778]

Immunoprecipitation: SGK1 Antibody [NBP2-98778] - SGK1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 ul anti-SGK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-SGK1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa
SGK1 Antibody - Azide and BSA Free

Western Blot: SGK1 Antibody [NBP2-98778] -

Western Blot: SGK1 Antibody [NBP2-98778] - NBP2-98778 at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:49 kDa Observed band size:49 kDa

Applications for SGK1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SGK1

SGK1 encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.

Long Name

Serum/Glucocorticoid Regulated Kinase 1

Alternate Names

SGK

Gene Symbol

SGK1

Additional SGK1 Products

Product Documents for SGK1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SGK1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

mTOR Signaling Pathway mTOR Signaling Pathway Thumbnail