SGSM2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SGSM2 Antibody [NBP1-93637]

Immunocytochemistry/Immunofluorescence: SGSM2 Antibody [NBP1-93637] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Immunohistochemistry-Paraffin: SGSM2 Antibody [NBP1-93637]

Immunohistochemistry-Paraffin: SGSM2 Antibody [NBP1-93637] - Staining of human cerebral cortex shows cytoplasmic positivity in neuronal and glia cells.

Immunohistochemistry: SGSM2 Antibody - BSA Free [NBP1-93637] -

SGSM2 expression was detected in human breast tissues and human breast cancer cell lines. (a) SGSM2 mRNA expressions in normal and malignant tissues was determined in 53 BC patients via RT-PCR, and the gel band intensities were quantified with PhotoCaptMw software (version 11.0.). We used normalized SGSM2 expression to classify patients into three groups: T > N, N = T, and N > T (N = normal tissue, and T = tumour tissue); and further, (b) quantitative real-time PCR was used to evaluate SGSM2 mRNA expression profiles in paired human breast tumour (red lines) and normal (green lines) tissues (n = 200). (c) The means of SGSM2 mRNA copy number (per μg of mRNA) were calculated from real-time PCR data. The data were analysed with a paired sample t-test with two-sided P-values. (d) In vitro SGSM2 and SGSM3 protein expression was detected in twelve breast cancer cell lines and one breast epithelial cell line; the mean density of SGSM2 and SGSM3 protein level in each cell line was normalized to beta -actin, and the values were compared to the MCF-10A cell line. The hormone receptor status is displayed at the bottom. (e) The distribution of SGSM2 and SGSM3 proteins were determined in ER+/ HER2 – breast cancer tissue sections; Haematoxylin (violet, for nuclei) and eosin (pink) (H&E) staining is shown in the left panels; anti-SGSM2 and anti-SGSM3 antibody bound to antigen is shown in the middle and right panels (N = normal region, T = tumour region). Top panels: 100x, scale bar: 100 μm; bottom panels: 400x, scale bar: 25 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30744493), licensed under a CC-BY license. Not internally tested by Novus Biologicals.