|Detection of SheepIgG Control by Flow Cytometry Human peripheral blood monocytes were stained with SheepAnti-Human IRF5 Alexa Fluor® 700‑conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC4508N,filled histogram) or Sheep IgG Alexa Fluor® 700-conjugatedIsotype Control Antibody (Catalog # IC016N, open histogram). Tofacilitate intracellular staining, cells were fixed with Flow CytometryFixation Buffer (Catalog # FC004)and permeabilized with Flow CytometryPermeabilization/Wash Buffer I (Catalog # FC005). View our protocol for StainingIntracellular Molecules.|
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.