Key Product Details

Species Reactivity

Sheep

Applications

Control

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG
View all IgG Isotype Controls »

Product Specifications

Specificity

Serum was obtained from naive (non-immunized) sheep.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Normal Sheep IgG Fluorescein Control

Detection of Sheep IgG Isotype Control by Flow Cytometry

Detection of Sheep IgG Control by Flow Cytometry

HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or Sheep IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Normal Sheep IgG Fluorescein Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 1 review rated 5 using IC016F in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Normal Sheep IgG Fluorescein Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Normal Sheep IgG Fluorescein Control

For research use only

Citations for Normal Sheep IgG Fluorescein Control

Customer Reviews for Normal Sheep IgG Fluorescein Control (1)

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  • Normal Sheep IgG Fluorescein Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Adult splenocytes
    Species: Mouse
    Verified Customer | Posted 08/24/2017
    Isotype control

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