hh Normal Sheep IgG Fluorescein Control IC016F: R&D Systems

Normal Sheep IgG Fluorescein Control

(1 Review)
  
  • Specificity
    Serum was obtained from naive (non-immunized) sheep.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Protein A or G purified
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Sheep IgGControl by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stainedwith Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated AntigenAffinity-purified Polyclonal Antibody (Catalog #IC5868F,filled histogram) or Sheep IgG Fluorescein-conjugated Isotype ControlAntibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cellswere fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/WashBuffer I (Catalog #FC005).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Sheep IgG APC-conjugated Antibody

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Sheep IgG PE-conjugated Antibody

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Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Sheep IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC016G  
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Sheep IgG Alexa Fluor® 647-conjugated Antibody

Ctrl IC016R  
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Sheep IgG Alexa Fluor® 700‑conjugated Control Antibody

Ctrl IC016N  
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Normal Sheep IgG Fluorescein Control.

We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Mouse Anonymous 08/24/2017
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Summary

ApplicationFlow Cytometry
Sample TestedAdult splenocytes
SpeciesMouse

Other Experimental Details

Other Experimental DetailsIsotype control

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