Normal Sheep IgG Fluorescein Control Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Sheep IgGControl by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stainedwith Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated AntigenAffinity-purified Polyclonal Antibody (Catalog #IC5868F,filled histogram) or Sheep IgG Fluorescein-conjugated Isotype ControlAntibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cellswere fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/WashBuffer I (Catalog #FC005).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
No product specific FAQs exist for this product, however you mayView all Isotype Control FAQs
Reviews for Normal Sheep IgG Fluorescein Control
Average Rating: 5 (Based on 1 Review)
Have you used Normal Sheep IgG Fluorescein Control?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image