Sheep IgG NorthernLights™ NL493-conjugated Antibody

NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL012

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Detection of Sheep IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Sheep IgG NorthernLights™ NL493-conjugated Antibody Summary

Species Reactivity
Sheep
Specificity
Sheep IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Sheep IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Use at a 1:200 dilution
 
Immunohistochemistry
Use at a 1:200 dilution
 
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Sheep IgG Primary Antibody by Immunocytochemistry. Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in KG-1 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Sheep IgG NorthernLights™ NL493-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells
    Authors: Ryoo, H;Underhill, GH;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays
    Authors: MS Szymczak, WK Reeves, MM Miller
    Viruses, 2021-08-30;13(9):.
    Species: Cercopithecus aethiops
    Sample Types: Whole Cells
    Applications: ICC
  3. Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix
    Authors: T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour
    Sci Rep, 2018-04-20;8(1):6331.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Inhibition of VEGF-dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC14a
    Authors: TK Kim, CS Park, J Jang, MR Kim, HJ Na, K Lee, HJ Kim, K Heo, BC Yoo, YM Kim, JW Lee, SJ Kim, ES Kim, DY Kim, K Cha, TG Lee, S Lee
    Mol Oncol, 2018-01-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro.
    Authors: Ki, M K, Jeoung, M H, Choi, J R, Rho, S-S, Kwon, Y-G, Shim, H, Chung, J, Hong, H J, Song, B D, Lee, S
    Oncogene, 2013-05-06;32(48):5449-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. HIV-1 harboring renal tubular epithelial cell interaction with T cells results in T cell trans-infection.
    Authors: Mikulak J, Teichberg S, Faust T, Schmidtmayerova H, Singhal PC
    Virology, 2008-12-17;385(1):105-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Sheep IgG NorthernLights™ NL493-conjugated Antibody

Average Rating: 3 (Based on 1 Review)

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody
By Anonymous on 07/27/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: sheep IgG Species: Sheep