SIAH2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-85730

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all SIAH2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen is a synthetic peptide directed towards the N-terminal region of human SIAH2. Peptide sequence: SRPSSTGPSANKPCSKQPPPQPQHTPSPAAPPAAATISAAGPGSSAVPAA The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for SIAH2 Antibody - BSA Free

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730] - Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH2. Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena.
Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730] - WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate
Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: HepG2. Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2
Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7
Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Tissue: Human OVCAR-3 Whole Cell. Antibody Dilution: 3ug/ml
Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730]

Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: Hela. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12

Applications for SIAH2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIAH2

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila, SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Alternate Names

E3 ubiquitin-protein ligase SIAH2, EC 6.3.2, EC 6.3.2.-, hSiah2, seven in absentia (Drosophila) homolog 2, Seven in absentia homolog 2, seven in absentia homolog 2 (Drosophila), Siah-2

Gene Symbol

SIAH2

Additional SIAH2 Products

Product Documents for SIAH2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SIAH2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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