Signal Peptide Peptidase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32276

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Signal Peptide Peptidase Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 327 to 377 of human Signal Peptide Peptidase Like 1 using the numbering given in entry NP_110416.1 (GeneID 81502).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Signal Peptide Peptidase Antibody - BSA Free

Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276]

Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276]

Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SPP antibody NBP2-32276 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276]

Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276]

Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-SPP antibody NBP2-32276 used for IP at 6 ug per reaction. SPP was also immunoprecipitated by rabbit anti-SPP antibody BL16569. For blotting immunoprecipitated SPP, NBP2-32276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Signal Peptide Peptidase Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Signal Peptide Peptidase

Signal Peptide Peptidase is encoded by this gene, which localizes to the endoplasmic reticulum, catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein. This activity is required to generate signal sequence-derived human lymphocyte antigen-E epitopes that are recognized by the immune system, and to process hepatitis C virus core protein. The encoded protein is an integral membrane protein with sequence motifs characteristic of the presenilin-type aspartic proteases. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

dJ324O17.1, EC 3.4.23.-, H13hIMP1, histocompatibility (minor) 13, IMP-1, IMP1MSTP086, Intramembrane protease 1, minor histocompatibility antigen 13, minor histocompatibility antigen H13, Presenilin-like protein 3, PSENL3, PSL3IMPAS, Signal peptide peptidase, signal peptide peptidase beta, SPPIMPAS-1

Gene Symbol

HM13

UniProt

NP_110416.1 (Human)

Additional Signal Peptide Peptidase Products

Product Documents for Signal Peptide Peptidase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Signal Peptide Peptidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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