Simple Plex Discovery Human Relaxin-2 Assay
R&D Systems | Catalog # SPCKB-PS-012704
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Relaxin-2 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 27 | 27 |
| Mean (pg/mL) | 5.39 | 236 | 4.93 | 210 |
| Standard Deviation | 0.155 | 8.72 | 0.406 | 17.2 |
| CV% | 2.9 | 3.7 | 8.2 | 8.2 |
Recovery for Simple Plex Discovery Human Relaxin-2 Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 107 | 105-109 |
| EDTA Plasma (n=2) | 102 | 92-111 |
| Heparin Plasma (n=2) | 114 | 110-123 |
| Serum (n=2) | 111 | 103-118 |
Linearity
Samples containing and/or spiked with high concentrations of human Relaxin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Relaxin-2 Assay
Simple Plex Discovery Human Relaxin-2 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Relaxin-2
Relaxin-2, also called H2 relaxin, is a peptide hormone that plays an important role in female reproduction. It circulates in the blood as a disulfide-linked dimer of alpha and beta chains. Relaxin is best known as a hormone of parturition that promotes growth and softening of the cervix, development of the mammary gland, and has a marked impact on the uterus. In particular, it promotes angiogenesis, inhibits MMP production and activity, and downregulates estrogen receptor-alpha expression. Relaxin-2 confers its activity by binding to leucine-rich guanine nucleotide-binding (G-protein) coupled receptors, LGR7 and LGR8.
Additional Relaxin-2 Products
Product Documents for Simple Plex Discovery Human Relaxin-2 Assay
Certificate of Analysis
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Product Specific Notices for Simple Plex Discovery Human Relaxin-2 Assay
For research use only
Related Research Areas
Citations for Simple Plex Discovery Human Relaxin-2 Assay
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