Simple Plex Discovery Human CCL11/Eotaxin Assay
R&D Systems | Catalog # SPCKB-PS-014624
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human CCL11/Eotaxin Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Eotaxin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Eotaxin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Eotaxin.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 24 | 24 |
| Mean (pg/mL) | 6.91 | 349 | 7.68 | 374 |
| Standard Deviation | 0.255 | 18.5 | 0.527 | 19.7 |
| CV% | 3.7 | 5.3 | 6.9 | 5.3 |
Recovery for Simple Plex Discovery Human CCL11/Eotaxin Assay
Recovery for the human Eotaxin assays was evaluated at three different spiked concentrations of Eotaxin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 108 | 108-109 |
Linearity
Samples containing and/or spiked with high concentrations of Eotaxin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human CCL11/Eotaxin Assay
Simple Plex Discovery Human Eotaxin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Eotaxin Assay. Assay range is 0.62-944 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL11/Eotaxin
CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.
Additional CCL11/Eotaxin Products
Product Documents for Simple Plex Discovery Human CCL11/Eotaxin Assay
Certificate of Analysis
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Product Specific Notices for Simple Plex Discovery Human CCL11/Eotaxin Assay
For research use only
Related Research Areas
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