Simple Plex Discovery Human CCL11/Eotaxin Assay New

Catalog #: SPCKB-PS-014624 Datasheet / COA / SDS

Simple Plex Human Eotaxin assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-014624
SPCKA-PS-014624
SPCKC-PS-014624

Control Products Available

897697: Simple Plex Discovery Control for Human CCL11/Eotaxin

Simple Plex Discovery Human Eotaxin Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human CCL11/Eotaxin Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.075 pg/mL

Assay Range

0.6 - 944 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Eotaxin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human CCL11/Eotaxin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Eotaxin assay

Inter-Assay Precision (Precision between assays) Replicates of each Eotaxin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Eotaxin

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	24
Mean (pg/mL)	6.91	349	7.68	374
Standard Deviation	0.255	18.5	0.527	19.7
CV%	3.7	5.3	6.9	5.3

Recovery

Recovery for the human Eotaxin assays was evaluated at three different spiked concentrations of Eotaxin protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	108	108-109

Linearity

Samples containing and/or spiked with high concentrations of Eotaxin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human Eotaxin Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Eotaxin Assay. Assay range is 0.62-944 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL11/Eotaxin

CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.

Entrez Gene IDs:

6356 (Human); 20292 (Mouse)

Alternate Names:

C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)