Simple Plex Discovery Human IL-15R alpha Cartridge
R&D Systems | Catalog # SPCKB-PS-016662
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human IL-15R alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL 15 R alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL 15 R alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL 15 R alpha.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 28 | 28 |
| Mean (pg/mL) | 33.8 | 1796 | 32.9 | 1680 |
| Standard Deviation | 1.34 | 67.6 | 2.77 | 128 |
| CV% | 4.0 | 3.8 | 8.4 | 7.6 |
Recovery for Simple Plex Discovery Human IL-15R alpha Cartridge
Recovery for the human IL 15 R alpha assays was evaluated at three different spiked concentrations of IL 15 R alpha protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 112 | 110-113 |
| EDTA Plasma (n=2) | 92 | 89-95 |
| Heparin Plasma (n=2) | 112 | 112-112 |
| Serum (n=2) | 107 | 105-109 |
Linearity
Samples containing and/or spiked with high concentrations of IL 15 R alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human IL-15R alpha Cartridge
Simple Plex Discovery Human IL 15 R alpha Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human IL 15 R alpha Assay. Assay range is 7.86-12,000 pg/mL.
Simple Plex Discovery Human IL 15 R alpha Assay Endogenous Levels
Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.Preparation and Storage
Shipping
Stability & Storage
Background: IL-15R alpha
IL-15Ra (Interleukin 15 Receptor alpha), also known as CD215, is a transmembrane glycoprotein that plays an important role in NK and CD8+ memory T cell homeostasis and activation. It transmits IL-15 induced signals and additionally interacts with a complex of IL-2Rβ and the common gamma chain (γc) which are also subunits of the IL-2 receptor complex. In transpresentation, IL-15/IL-15Ra complexes are expressed on the surface of one cell and interact with complexes of IL-2Rb/gc on adjacent cells. IL-15/IL-15Ra complexes can transmit reverse signaling that promotes adhesion and activation of the IL-15/IL-15Ra expressing cells. Shed soluble forms of IL-15Ra retain the ability to bind tightly to IL-15 and can inhibit IL-15 bioactivity.
Long Name
Alternate Names
Gene Symbol
Additional IL-15R alpha Products
Product Documents for Simple Plex Discovery Human IL-15R alpha Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Discovery Human IL-15R alpha Cartridge
For research use only
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