Simple Plex Discovery Human IL-3 Assay New

Catalog #: SPCKB-PS-012017 Datasheet / COA / SDS

Simple Plex Human IL-3 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012017
SPCKA-PS-012017
SPCKC-PS-012017

Control Products Available

897180: Simple Plex Discovery Control for Human IL-3

Simple Plex Discovery Human IL-3 Ella Assay

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All IL-3 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human IL-3 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.4 pg/mL

Assay Range

1.8 - 2,728 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Interleukin 3 (IL-3) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	23
Mean (pg/mL)	21.3	1318	19.8	1238
Standard Deviation	1.17	83.2	2.28	98.1
CV%	5.5	6.3	11.5	7.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	110	109-112
EDTA Plasma (n=2)	81	79-91
Heparin Plasma (n=2)	96	91-101
Serum (n=2)	79	70-87

Linearity

Samples containing and/or spiked with high concentrations of recombinant human IL-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human IL-3 Ella Assay Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-3

Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.

Long Name:

Interleukin 3

Entrez Gene IDs:

3562 (Human); 16187 (Mouse); 24495 (Rat)

Alternate Names:

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor