Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay

R&D Systems | Catalog # SPCKB-PS-014625

Simple Plex Human OX40 Ligand assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.58 pg/mL

Assay Range

3.14-12000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay Summary

Simple Plex Discovery assay is for the detection of human OX40 Ligand/TNFSF4 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all OX40 Ligand/TNFSF4 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human OX40 Ligand.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one OX40 Ligand assay.

Inter-Assay Precision (Precision between assays) Replicates of each OX40 Ligand control were tested in multiple assays performed by at least three technicians using two lots of reagents for human OX40 Ligand.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 32 33
Mean (pg/mL) 156 7307 92.5 3910
Standard Deviation 11.9 475 8.00 335
CV% 7.6 6.5 8.7 8.6

Recovery for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay

Recovery for the human OX40 Ligand assays was evaluated at three different spiked concentrations of OX40 Ligand protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 104 91-117
EDTA Plasma (n=2) 85 74-95
Heparin Plasma (n=2) 100 95-104
Serum (n=2) 90 86-95

Linearity

Samples containing and/or spiked with high concentrations of OX40 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human OX40 Ligand Assay Linearity

Scientific Data Images for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay

Simple Plex Discovery Human OX40 Ligand Assay Standard Curve

Simple Plex Discovery Human OX40 Ligand Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human OX40 Ligand Assay. Assay range is 3.14-12,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a member of the TNF superfamily. Human OX40L shares 46% amino acid sequence identity with its mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similar to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily, which is expressed predominantly on activated CD4+ T cells.

Alternate Names

CD252, gp34, TNFSF4

Entrez Gene IDs

7292 (Human); 22164 (Mouse); 89814 (Rat)

Gene Symbol

TNFSF4

Additional OX40 Ligand/TNFSF4 Products

Product Documents for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human OX40 Ligand/TNFSF4 Assay

For research use only

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