Simple Plex Discovery Human PDGF-AB Assay
R&D Systems | Catalog # SPCKB-PS-012303
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human PDGF-AB Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 31 | 31 |
| Mean (pg/mL) | 83.9 | 4019 | 70.5 | 3466 |
| Standard Deviation | 3.30 | 108 | 6.81 | 292 |
| CV% | 3.9 | 2.7 | 9.7 | 8.4 |
Recovery for Simple Plex Discovery Human PDGF-AB Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 113 | 112-115 |
Linearity
Samples containing and/or spiked with high concentrations of human PDGF-AB were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human PDGF-AB Assay
Simple Plex Discovery Human PDGF-AB Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: PDGF-AB
PDGF-A and PDGF-B are platelet-derived growth factors that can form disulfide-linked homodimers or heterodimers with each other. These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of PDGF R alpha and R beta. PDGF-AB binds to alpha receptor homodimers as well as the heterodimeric receptor. PDGF-AA and -AB promote cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. They are important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles, and intestinal villus.
Long Name
Alternate Names
Additional PDGF-AB Products
Product Documents for Simple Plex Discovery Human PDGF-AB Assay
Certificate of Analysis
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Product Specific Notices for Simple Plex Discovery Human PDGF-AB Assay
For research use only
Related Research Areas
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