Simple Plex Discovery Human Relaxin-2 Assay New

Catalog #: SPCKB-PS-012704 Datasheet / COA / SDS

Simple Plex Human Relaxin-2 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012704
SPCKA-PS-012704
SPCKC-PS-012704

Control Products Available

898218: Simple Plex Discovery Control for Human Relaxin-2

Simple Plex Discovery Human Relaxin-2 Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Relaxin-2 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.133 pg/mL

Assay Range

0.4 - 576 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Relaxin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Relaxin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	27	27
Mean (pg/mL)	5.39	236	4.93	210
Standard Deviation	0.155	8.72	0.406	17.2
CV%	2.9	3.7	8.2	8.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	107	105-109
EDTA Plasma (n=2)	102	92-111
Heparin Plasma (n=2)	114	110-123
Serum (n=2)	111	103-118

Linearity

Samples containing and/or spiked with high concentrations of human Relaxin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human Relaxin-2 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Relaxin-2

The Insulin/Relaxin superfamily includes Insulin, IGF-I, IGF-II, Relaxins, Relaxin-like Factor (RLF), Early Placenta Insulin-like Peptide 4 (INSL4), INSL5, and INSL6. Three human Relaxin genes (Relaxins-1 to -3) have been identified. Relaxin-2 is secreted and circulates in the blood, whereas Relaxin-1 is expressed in decidua, placenta, and prostate, and Relaxin-3 is expressed in the brain. Human Relaxin-2 is a peptide hormone that plays an important role in female reproduction.

Entrez Gene IDs:

6019 (Human)

Alternate Names:

bA12D24.1.1; bA12D24.1.2; H2; relaxin 2; Relaxin2; Relaxin-2; RLN2; RLXH2