Simple Plex Human ASC Cartridge

R&D Systems | Catalog # SPCKB-PS-003913

Simple Plex Human ASC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.95 pg/mL

Assay Range

5.24-8000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human ASC Cartridge Summary

Simple Plex assay is for the detection of human Apoptosis-associated speck-like protein containing a CARD (ASC) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all ASC Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human ASC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ASC assay.

Inter-Assay Precision (Precision between assays) Replicates of each ASC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ASC.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 57 55
Mean (pg/mL) 65.2 3191 61.4 3062
Standard Deviation 3.21 170 6.23 294
CV% 4.9 5.3 10.1 9.6

Recovery for Simple Plex Human ASC Cartridge

Recovery was not necessary as human ASC shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of ASC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human ASC Assay Linearity

Scientific Data Images for Simple Plex Human ASC Cartridge

Simple Plex Human ASC Assay Standard Curve

Simple Plex Human ASC Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human ASC Assay. Assay range is 5.24-8000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ASC

ASC contains an N-terminal Pyrin domain and C-terminal CARD (Caspase-recruitment Domain), and is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli. Methylation of the ASC gene has been correlated with decreased protein expression in numerous types of cancer.

Long Name

Apoptosis-Associated Speck-Like Protein Containing A CARD

Alternate Names

CARD5, PYCARD, TMS1

Entrez Gene IDs

29108 (Human)

Gene Symbol

PYCARD

Additional ASC Products

Product Documents for Simple Plex Human ASC Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human ASC Cartridge

For research use only

Citations for Simple Plex Human ASC Cartridge

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