Simple Plex Human BMP-2 Cartridge

R&D Systems | Catalog # SPCKB-PS-000245

Simple Plex Human BMP-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

8.36 pg/mL

Assay Range

19.7-30000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human BMP-2 Cartridge Summary

Simple Plex assay is for the detection of human B-Lymphocyte Chemoattractant (BMP-2) in serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all BMP-2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human BMP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BMP-2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each BMP-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BMP-2.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 28 24
Mean (pg/mL) 170 8191 174 8201
Standard Deviation 12.8 636 16.9 541
CV% 7.5 7.8 9.7 6.6

Recovery for Simple Plex Human BMP-2 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 87-107
Heparin Plasma (n=4) 93 83-106
Serum (n=4) 94 88-98

Linearity

Samples containing and/or spiked with high concentrations of human BMP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human BMP-2 Assay Linearity

Scientific Data Images for Simple Plex Human BMP-2 Cartridge

Simple Plex Human BMP-2 Assay Standard Curve

Simple Plex Human BMP-2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BMP-2 Assay. Assay range is 19.7-30,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-2

BMP-2 (Bone morphogenetic protein 2) is a TGF-beta superfamily cytokine that plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 forms disulfide linked homodimers and heterodimers with BMP-7. BMP-2 signals through heterodimeric complexes of type I receptors (ActiRI, BMPR-IA, or BMPR-IB) and type II receptors (BMP RII or ActRIIB). It induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair. BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.

 

Long Name

Bone Morphogenetic Protein 2

Alternate Names

BDA2, BMP2, SSFSC

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

Additional BMP-2 Products

Product Documents for Simple Plex Human BMP-2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human BMP-2 Cartridge

For research use only

Citations for Simple Plex Human BMP-2 Cartridge

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