Simple Plex Human CCL17/TARC Cartridge

Catalog #: SPCKB-PS-000194 Datasheet / COA / SDS
Simple Plex Human CCL17/TARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000194
SPCKA-PS-000194
SPCKC-PS-000194
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Human TARC Simple Plex Assay
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Simple Plex Human CCL17/TARC Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.418 pg/mL
Assay Range
0.6 - 850 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human TARC.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human TARC in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) control was Each tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 14 13
Mean (pg/mL) 6.33 417 6.84 393
Standard Deviation 0.298 38.9 0.541 41.8
CV% 4.7 9.3 7.9 10.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 84-112
Heparin Plasma (n=4) 97 79-110
Serum (n=4) 115 98-122

Linearity

Samples containing and/or spiked with high concentrations of human TARC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human TARC Simple Plex Assay Linearity

Scientific Data

Human TARC Simple Plex Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL17/TARC

CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.

Entrez Gene IDs:
6361 (Human); 20295 (Mouse)
Alternate Names:
ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

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