Simple Plex Human CCL17/TARC Cartridge

Name: Simple Plex Human CCL17/TARC Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000194
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000194

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Product Documents

Simple Plex Human CCL17/TARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.418 pg/mL

Assay Range

0.56-850 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CCL17/TARC Cartridge Summary

Simple Plex assay for the detection of human Thymus and Activationo-Regulated Chemokine (TARC) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TARC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	14	13
Mean (pg/mL)	6.33	417	6.84	393
Standard Deviation	0.298	38.9	0.541	41.8
CV%	4.7	9.3	7.9	10.6

Recovery for Simple Plex Human CCL17/TARC Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	109	107-111
EDTA Plasma (n=4)	99	84-112
Heparin Plasma (n=4)	97	79-110
Serum (n=4)	115	98-122

Linearity

Samples containing and/or spiked with high concentrations of human TARC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human CCL17/TARC Cartridge

Human CCL17/TARC Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL17/TARC

CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.