Simple Plex Human CCL22/MDC Cartridge

Name: Simple Plex Human CCL22/MDC Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000866
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000866

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Product Documents

Simple Plex Human CCL22/MDC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.32 pg/mL

Assay Range

0.68-2600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CCL22/MDC Cartridge Summary

Simple Plex assay is for the detection of human Macrophage-Derived Chemokine (CCL22/MDC) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CCL20/MDC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CCL22/MDC assay.

Inter-Assay Precision (Precision between assays) Replicates of each CCL22/MDC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CCL22/MDC.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	17
Mean (pg/mL)	17.2	975	17.5	890
Standard Deviation	0.947	68.6	1.23	49.9
CV%	5.5	7.0	7.0	5.6

Recovery for Simple Plex Human CCL22/MDC Cartridge

Recovery for the human CCL22/MDC assays was evaluated at three different spiked concentrations of CCL22/MDC protein.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	102	87-108

Linearity

Samples containing and/or spiked with high concentrations of human CCL22/MDC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human CCL22/MDC Cartridge

Simple Plex Human CCL22/MDC Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CCL22/MDC Assay. Assay range is 0.68-2600 pg/mL.

Background: CCL22/MDC

Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.