Simple Plex Human CCL3/MIP-1 alpha Cartridge

Name: Simple Plex Human CCL3/MIP-1 alpha Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000476
Price: 600 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000476

Submit a review

Citations

Product Documents

Simple Plex Human CCL3/MIP-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.5 pg/mL

Assay Range

1.05-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CCL3/MIP-1 alpha Cartridge Summary

Simple Plex assay is for the detection of human Macrophage Inflammatory Protein 1 alpha (MIP-1 alpha ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CCL3/MIP-1 alpha Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MIP-1 alpha.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MIP-1 alpha assay.

Inter-Assay Precision (Precision between assays) Replicates of each MIP-1 alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MIP-1 alpha.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (pg/mL)	22.5	1222	21.8	1179
Standard Deviation	1.05	58.7	0.910	46.1
CV%	4.7	4.8	4.2	3.9

Recovery for Simple Plex Human CCL3/MIP-1 alpha Cartridge

Recovery for the human MIP-1 alpha assays was evaluated at three different spiked concentrations of MIP-1 alpha protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	89-99
Heparin Plasma (n=4)	93	90-99
Serum (n=4)	97	93-100

Linearity

Samples containing and/or spiked with high concentrations of MIP-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human CCL3/MIP-1 alpha Cartridge

Simple Plex Human MIP-1 alpha Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL3/MIP-1 alpha

CCL3/MIP-1 alpha, is a beta chemokine that can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells. It forms complexes with sulfated proteoglycans and also associates into noncovalently-linked dimers and high molecular weight polymers. These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine. CCL3 signals through CCR1, CCR3, and CCR5, and it is internalized by the decoy chemokine receptor D6. CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types. It can promote tumor metastasis, hyperalgesia, and inflammation and suppress HIV replication and hematopoietic stem cell proliferation.