Simple Plex Human CD40/TNFRSF5 Cartridge

Name: Simple Plex Human CD40/TNFRSF5 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000196
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000196

Submit a review

Citations

Product Documents

Simple Plex Human CD40/TNFRSF5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.664 pg/mL

Assay Range

4.28-16324 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CD40/TNFRSF5 Cartridge Summary

Simple Plex assay is for the detection of human CD40 in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CD40/TNFRSF5 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CD40.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD40 assay.

Inter-Assay Precision (Precision between assays) Replicates of each CD40 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD40.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	16
Mean (pg/mL)	226	11,174	230	11,840
Standard Deviation	6.90	526	9.92	973
CV%	3.0	4.7	4.3	8.2

Recovery for Simple Plex Human CD40/TNFRSF5 Cartridge

Recovery was not necessary as human CD40 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human CD40 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human CD40/TNFRSF5 Cartridge

Simple Plex Human CD40 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD40 Assay. Assay range is 4.28-16,324 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a transmembrane TNF superfamily receptor that is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. Interaction of CD40 with CD40 Ligand leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells. This interaction promotes B cell activation and T cell-dependent humoral responses. CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy. Dysregulation of CD40/CD40 Ligand expression and interactions contributes to disease severity in AIDS, atherosclerosis, atherothrombosis, and restenosis.