Simple Plex Human CTLA-4 Cartridge

R&D Systems | Catalog # SPCKB-PS-003114

Simple Plex Human CTLA-4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.1 pg/mL

Assay Range

3.14-30000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CTLA-4 Cartridge Summary

Simple Plex assay is for the detection of human Cytotoxic T-Lymphocyte Associated protein-4 (CTLA-4) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CTLA-4 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CTLA-4.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CTLA-4 assay.

Inter-Assay Precision (Precision between assays) Replicates of each CTLA-4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CTLA-4.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 54 51
Mean (pg/mL) 209 9821 212 9517
Standard Deviation 8.97 616 16.4 701
CV% 4.3 6.3 7.7 7.4

Recovery for Simple Plex Human CTLA-4 Cartridge

Recovery for the human CTLA-4 assays was evaluated at three different spiked concentrations of CTLA-4 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 89 83-99
Heparin Plasma (n=4) 87 84-90
Serum (n=4) 95 89-100

Linearity

Samples containing and/or spiked with high concentrations of human CTLA-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human CTLA-4 Assay Linearity

Scientific Data Images for Simple Plex Human CTLA-4 Cartridge

Simple Plex Human CTLA-4 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CTLA-4 Assay. Assay range is 3.14-30,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CTLA-4

CTLA-4/CD152 (Cytotoxic T-Lymphocyte Antigen 4) is a transmembrane T cell inhibitory molecule that is expressed as a covalent homodimer. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending CD28-dependent T cell activation. Mice deleted for CTLA-4 develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells which constitutively express CTLA-4.

Long Name

Cytotoxic T-lymphocyte-associated Molecule 4

Alternate Names

CD152, CTLA4

Entrez Gene IDs

1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)

Gene Symbol

CTLA4

Additional CTLA-4 Products

Product Documents for Simple Plex Human CTLA-4 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human CTLA-4 Cartridge

For research use only

Citations for Simple Plex Human CTLA-4 Cartridge

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